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pet32a (+) vector  (Thermo Fisher)


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    Structured Review

    Thermo Fisher pet32a (+) vector
    Pet32a (+) Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pet32a+(+)+vector/pm39724843-117-5-8
    Average 90 stars, based on 1 article reviews
    pet32a (+) vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Activation of protein arginine methyltransferase 1 and subsequent extension of moth lifespan is effected by the ROS/JNK/CREB signaling axis
    Article Snippet: .. Partial ORFs of Har-JNK , Har-FoxO , Har-CREB , Har-PRMT1 , and Har-Actin were amplified and cloned into the pET32a vector (Invitrogen). ..

    Article Title: CBR1 decreases protein carbonyl levels via the ROS/Akt/CREB pathway to extend lifespan in the cotton bollworm, Helicoverpa armigera.
    Article Snippet: doi:10.1111/febs.16691 Reactive oxygen species (ROS) are considered a major cause of ageing and ageing-related diseases through protein carbonylation.. Little is known about the molecular mechanisms that confer protection against ROS.. Here, we observed that, compared with nondiapause-destined pupae, high protein carbonyl levels are present in the brains of diapause-destined pupae, which is a ‘non-ageing’ phase in the moth Helicoverpa armigera.

    Article Title: FoxO induces pupal diapause by decreasing TGFβ signaling
    Article Snippet: .. Partial ORFs (open reading frames) of Har-FoxO, Har-TGFβRI and Har-Actin were amplified and cloned into the pET32a vector (Invitrogen). ..

    Clone Assay:

    Article Title: Activation of protein arginine methyltransferase 1 and subsequent extension of moth lifespan is effected by the ROS/JNK/CREB signaling axis
    Article Snippet: .. Partial ORFs of Har-JNK , Har-FoxO , Har-CREB , Har-PRMT1 , and Har-Actin were amplified and cloned into the pET32a vector (Invitrogen). ..

    Article Title: Physiological mitochondrial ROS regulate diapause by enhancing HSP60/Lon complex stability in Helicoverpa armigera
    Article Snippet: .. Briefly, partial sequences of Har-Actin and Har-HSP60 were cloned into the pET32a vector (Invitrogen). .. The recombinant proteins of Har-HSP60 and Har-Actin were expressed in BL21 (DE3) by induction with IPTG at 20°C for 9–12 h, and then purified on a NTA-Ni2+-agarose column (Qiagen, China).

    Article Title: FoxO induces pupal diapause by decreasing TGFβ signaling
    Article Snippet: .. Partial ORFs (open reading frames) of Har-FoxO, Har-TGFβRI and Har-Actin were amplified and cloned into the pET32a vector (Invitrogen). ..

    Plasmid Preparation:

    Article Title: Activation of protein arginine methyltransferase 1 and subsequent extension of moth lifespan is effected by the ROS/JNK/CREB signaling axis
    Article Snippet: .. Partial ORFs of Har-JNK , Har-FoxO , Har-CREB , Har-PRMT1 , and Har-Actin were amplified and cloned into the pET32a vector (Invitrogen). ..

    Article Title: Physiological mitochondrial ROS regulate diapause by enhancing HSP60/Lon complex stability in Helicoverpa armigera
    Article Snippet: .. Briefly, partial sequences of Har-Actin and Har-HSP60 were cloned into the pET32a vector (Invitrogen). .. The recombinant proteins of Har-HSP60 and Har-Actin were expressed in BL21 (DE3) by induction with IPTG at 20°C for 9–12 h, and then purified on a NTA-Ni2+-agarose column (Qiagen, China).

    Article Title: Whole-genome and genome-wide association studies improve key agricultural traits of safflower for industrial and medicinal use
    Article Snippet: .. The CtCGT1 gene was inserted into the pET32a vector (Thermo Fisher, USA) to enable prokaryotic expression. ..

    Article Title: Genome-wide screen and multi-omics analysis reveal OGT1 participate in the biosynthesis of safflower flavonoid glycosides
    Article Snippet: .. The prokaryotic expression of the CtOGT1 gene relied on the pET32a vector (Thermo Fisher, USA), with primers provided in Supplementary Table 1. ..

    Article Title: FoxO induces pupal diapause by decreasing TGFβ signaling
    Article Snippet: .. Partial ORFs (open reading frames) of Har-FoxO, Har-TGFβRI and Har-Actin were amplified and cloned into the pET32a vector (Invitrogen). ..

    Expressing:

    Article Title: Whole-genome and genome-wide association studies improve key agricultural traits of safflower for industrial and medicinal use
    Article Snippet: .. The CtCGT1 gene was inserted into the pET32a vector (Thermo Fisher, USA) to enable prokaryotic expression. ..

    Article Title: Genome-wide screen and multi-omics analysis reveal OGT1 participate in the biosynthesis of safflower flavonoid glycosides
    Article Snippet: .. The prokaryotic expression of the CtOGT1 gene relied on the pET32a vector (Thermo Fisher, USA), with primers provided in Supplementary Table 1. ..

    Article Title: Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM.
    Article Snippet: Nattokinase, a serine protease, was discovered in Bacillus subtilis during the fermentation of a soybean byproduct.. Nattokinase is essential for the lysis of blood clots and the treatment of cardiac diseases including atherosclerosis, thrombosis, high blood pressure, and stroke.. The demand for thrombolytic drugs rises as the prevalence of cardiovascular disease rises, and nattokinase is particularly effective for the treatment of cardiovascular diseases due to its long duration of action.

    Western Blot:

    Article Title: CBR1 decreases protein carbonyl levels via the ROS/Akt/CREB pathway to extend lifespan in the cotton bollworm, Helicoverpa armigera.
    Article Snippet: doi:10.1111/febs.16691 Reactive oxygen species (ROS) are considered a major cause of ageing and ageing-related diseases through protein carbonylation.. Little is known about the molecular mechanisms that confer protection against ROS.. Here, we observed that, compared with nondiapause-destined pupae, high protein carbonyl levels are present in the brains of diapause-destined pupae, which is a ‘non-ageing’ phase in the moth Helicoverpa armigera.

    Cloning:

    Article Title: Heterologous expression of recombinant nattokinase in Escherichia coli BL21(DE3) and media optimization for overproduction of nattokinase using RSM.
    Article Snippet: Nattokinase, a serine protease, was discovered in Bacillus subtilis during the fermentation of a soybean byproduct.. Nattokinase is essential for the lysis of blood clots and the treatment of cardiac diseases including atherosclerosis, thrombosis, high blood pressure, and stroke.. The demand for thrombolytic drugs rises as the prevalence of cardiovascular disease rises, and nattokinase is particularly effective for the treatment of cardiovascular diseases due to its long duration of action.



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